We offer innovative food allergen ELISA kits for gluten-free which can effectively solubilize and extract proteins from processed and unprocessed food by using an innovative new extraction solution.This Wheat / Gluten (Gliadin) ELISA kit is sandwich enzyme immunoassays for the quantitative determination of Gluten amount in processed or unprocessed food. This kit is validated in multi-laboratories (published in J. AOAC Int. 2006) and announced as a gluten analytical method in Gluten Free Food Labeling Rule established in August 2013 by US-FDA.
Using an innovative extraction solution, the recovery rate is extremely increased. The Wheat / Gluten (Gliadin) ELISA kit can detect the target protein from highly processed food.
The sensitivity of the gluten is 1.05 μg/g food. The dynamic range detectable by this kit is 1.05 – 68 ppm gluten (High Range Assay).
The specially prepared polyclonal antibody can achieve less cross-reactivity against the foods using various ingredient.
The sample preparation/extraction is performed by using standard extraction solution for all food allergen ELISA Kits.
|Principle||Two step Sandwich ELISA method|
|Measuring range||Recommended Assay : 0.26 – 17 ppm gluten
High Range Assay : 1.05 – 68 ppm gluten
|Sample volume||0.1 mL|
|Measurable sample number||24 samples (Triplicate)|
|Sample Extraction||Overnight (at least 12 hours) or Heat for 10min as Short Time Extraction Method|
|Measuring Time||Within 2 hours (excluding Sample Preparation/Extraction)|
|Reproducibility||Intra-, Inter-, Lot to Lot reproducibility: C.V.≦ 10%|
|Kit component||All liquid (except Antibody-coated module)|
|Storage||2 – 8 degree Celsius|
|Product Name||Target Protein||More Information|
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