Morinaga Institute of
Biological Science, Inc.

AOAC PTM approved test kit for gluten analysis

Wheat/Gluten ELISA Kit
Cat. M2103

Wheat/Gluten ELISA Kit Cat. M2103

Wheat/Gluten ELISA Kit is sandwich enzyme immunoassay for the quantitative determination of gluten amount in raw materials and processed or non-processed foods.

The test kit is approved by AOAC PTM (performance tested method).

In addition, the test kit is listed on the GFCO (Gluten-Free Certification Organization) test kits.

Specifications

Test Method ELISA
No. wells 96 wells / kit
Limit of detection 0.31 ppm [ µg of wheat protein / g of food ] / 0.26 ppm [ µg of gluten / g of food ]
Limit of quantification 0.31 ppm [ µg of wheat protein / g of food ] / 0.26 ppm [ µg of gluten / g of food ]
Target antigen Gliadin
Calculated result Wheat protein or Gluten
Range of assay 0.31 - 20 ppm for wheat protein, 026 - 17 ppm for gluten
Assay time 2 h
Storage temperature 2℃ - 8℃

Technical note

- why does our test kit can detect target proteins from both raw materials and processed foods?

Processed food contains a mix of native and denatured proteins
Technical note
Denatured proteins are insoluble and difficult to be detected by using popular buffer to extract
Technical note
The Morinaga kit detects both native and denatured target proteins, making it ideal for both raw materials and processed foods

Our original extraction buffer enables to solubilize denatured proteins.

It also allows native proteins to be a same conformation.

Technical note

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